Animated Films Edit
- Constance Nebbercracker: Blown up through the combined efforts of DJ, Chowder, and Jenny.
- Shaw: Gets beaten up and humiliated by the forest animals.
Live-Actions Films Edit
- Edgar the Bug: Blown apart internally by Agent K, then shot by Laurel Weaver.
- Norman Osborn: Attempts to kill Peter Parker (AKA Spider-Man) with his glider, but accidentally impales himself.
- Jarra: Outsmarted and killed by Agent J.
- Serleena: Destroyed by Agent J, respawns, then gets swallowed by Jeff the worm, and finally destroyed for good by J and K.
- Dr. Otto Octavius: Regains his senses and sacrifices himself to stop his deadly experiment and save the city.
- Flint Marko: Tells Peter what actually happened the night his uncle was killed before turning to sand and blowing away.
- Eddie Brock: Killed by one of Harry's bombs used by Peter to destroy the symbiote.
- Harry Osborn: Dies from his injuries after reconciling with Peter.
- Bowling Ball Head: Gets his head ripped off by Agent K, then used as a bowling ball by Agent J twice.
- Boris The Animal: Outsmarted and overpowered by Agent J (Future Boris), gets his left arm destroyed again and later killed by Agent K (Past Boris).
- Emil Stenz: Killed when a grenade belt tied around his throat detonates.
- Martin Walker: Shot to death by John Cale.
- Eli Raphelson: Exposed and arrested.